Animal experimentation was performed, in that researchers stated, by injecting cardiac myocytes which were extracted from the Human-induced pluripotent stem cells (hiPSCs), which is having a capacity to recover from a heart attack.
Regeneration Of Dead Heart Muscle Cells Using A New Technique
For this study, they took heart models of pigs, because pig’s hearts are quite similar in activities such as functioning and having similar dimensions, in simple words, it is similar to the physiology of the human heart. Pig’s heart shows more relevant information similar to humans when compared to mice.
Biomedical engineering analyst has shown that after heart failure, there is no regeneration of cardiac muscle tissue, that leads to tissue death and the Pathogenic enlargement occurs.
Now, researchers generated a new technique that will create new tissue for the replacement of necrosis by injecting heart muscle cells. Similarly, they have researched the division of heart cells that were present near the injured area. Infarction refers to dead tissue which is caused due to low supplement of oxygen (blood) to this affected site.
By injecting heart muscle cells to the affected area with approximately thirty Million biomedical engineered human heart muscle cells those were transformed from Human-induced pluripotent stem cells. CCND2 (Cyclin D2) is considered a protein-encoding gene, that is mainly involved in the division of cells. By comparing both human heart muscle cells and cyclin D2 heart muscle cells, it is clear that the healing process is high in the cyclin D2.
After injecting, cell proliferation and pathogenic enlargement are reduced, and also the function is improved, necrotic tissue dimensions are also reduced.
Interestingly, the stimulation of cyclin D2 heart muscle cells was done not only by its own expansion but also with the proliferation of Existent cardio-myocytes around the necrotic site of the pig’s heart and also the production of new blood vessels from the existing vessels also referred to as angiogenesis.
This study suggests that the repair of affected heart tissue can be done by using a new plan, that is, transplantation of cyclin D2 heart muscle cells. The proliferation from the pre-existing heart muscle cells can be done by the stimulation of cyclin D2 cardio-myocytes, this follows cell signaling mainly paracrine signaling belonging to cellular communication.
Exosomes are the prominent ones in this paracrine signaling. These exosomes contain Proteins and Ribonucleic acid (RNA). The exosomes undergo purification from the cyclin D2 heart muscle cells, which instantly promotes the proliferation of cultured heart muscle cells.
Besides, the cultured cardio-myocytes were more contrary towards a condition called apoptosis. These exosomes are also involved in the process of proliferation of different cell types. As we know, exosomes contain (information) microRNA (miRNA) – a noncoding RNA.
This miRNA can interact with the mRNA (Messenger RNA) in the targeted cells. Human beings consist of a huge number of miRNA (with dissimilar RNA sequences) those were approximately two thousand miRNAs, and these were participating in the regulation of genes. Congestive heart failure (CHF), Acute myocardial infarction can be treated as well prevented in the future by using this latest technique.